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1. Flowjo reference serial#
2. Flowjo reference update#
3. Flowjo reference manual#
4. Flowjo reference registration#
5. Flowjo reference software#

With FlowSOM in Cytobank, you can include up to 4 million events on Premium and 8 million events on Enterprise Cytobank with basic compute. The number of events that will be included (sampled) from each population/file is displayed next to the total available events and will be dependent on the next configuration step: event sampling. Note that samples designated as experimental controls will not appear for selection. Click on the filenames to choose the samples that should be included in the FlowSOM analysis. Regardless, we recommend manually gating to at least Live Singlets to allow for a cleaner representation of the data.Īfter a population is selected, a list of samples is visible under the FCS Files section. Other users may be interested in a single subset and selecting a more specific population (such as CD4+) will narrow the focus of the analysis. The correct population for a FlowSOM analysis will vary by experiment some users may want a more objective look at the data and using a more general population (such as CD45+ or Singlets) will provide this. If populations are missing from the list or event counts seem incorrect, make sure the most recent version of changes in the gating interface has been applied to the experiment.

Flowjo reference manual#

The populations available in this list are taken from manual gating done in the experiment on Cytobank, including any tailored gates. The first step in running a FlowSOM analysis is choosing one or more populations from which the events will be sourced, and which samples (i.e.

This webpage reviews how PIs can then manage that workstation within the lab account. To create a computer workstation within the lab for multiple users, PIs must contact core facility staff to enable this special feature.

Flowjo reference update#

The tab will then update to indicate that the device has been authorized. Otherwise, simply click the "FlowJo Portal Sign In" button at the top and enter your personal Portal account credentials.

Flowjo reference serial#

If your computer has been used previously using the prior HWA system, you will need to remove that serial license number located in the middle right of that tab.

Flowjo reference software#

Open FlowJo software and choose "Preferences" icon shaped like a heart on the upper right, and then the "License" button again on the upper right. It is necessary to use your Yale domain email address during your registration.Ģ. Earlier versions of FlowJo are not compatible with Portal access.ġ. If you are an investigator who wishes to use multiple devices and have already confirmed that your lab is participating, then please first ensure that you have downloaded v10.5 or later. If you are a PI and wish to establish a lab account, please contact Lesley Devine who will arrange the establishment of your account and send an activation link by email. Trainees who only wish to use a workstation can register a "free" account without obligating the lab to pay for their personal devices. A workstation license seat that is tied to that computer and allows anybody to use that workstation software so long as they have a email address and have registered. A personal license seat that entitles them to use FlowJo on up to 4 devices of their choice.Ģ. Pricing is dependent on the total number of users that register on campus. PIs will be charged monthly for the individuals and workstations associated with their lab account. The facility will administer this site license. Please contact core facility staff if continued use of v9 is important for your research. Please note that only v10.5 is compatible with this licensing system, however it is possible to arrange for access to earlier versions through FlowJo vendor staff. Instead, individual investigators now have the ability to use FlowJo on multiple personal devices on the same personal license seat. The older online system restricted a license seat to the hardware addresses of a single computer and is no longer being offered by FlowJo.

Flowjo reference registration#

Yale's site license for the new FlowJo Portal system uses an online registration system. As of September 1st 2020, YSM labs that use this core facility will have access to the FlowJo Portal system.